KlenTaq & KlenTaq LA

KlenTaq DNA Polymerase is a Taq polymerase variant that lacks the 5’-exonuclease activity due to an N-terminal deletion of Taq. This modification results in improved fidelity and thermostability compared to the wild-type Taq. Although not as strong as our new generation of KlenTaq or Taq mutants, KlenTaq does have some inhibition-resistant features.

KlenTaq DNA Polymerase is suitable for real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it cannot be used in real-time PCR TaqMan assays, which require 5’-exonuclease activity. This enzyme is inhibition-resistant as well and able to tolerate up 30% blood in PCR.

KlenTaq LA DNA Polymerase is the Long Accurate version of KlenTaq, a Taq polymerase variant that lacks 5’-exonuclease activity due to an N-terminal deletion of Taq. This modification results in improved fidelity and thermostability compared to the wild-type Taq. KlenTaq LA is designed to amplify longer gene targets compared to the standard KlenTaq polymerase.

KlenTaq LA DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it is not suitable for real-time PCR TaqMan assays that require 5’-exonuclease activity.

Highlights

• Thermostability - more thermostable and improved fidelity than Taq DNA polymerase
• Efficient – novel buffer system maximizes efficiency of PCR amplification, increases yield of any PCR product
• Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
• Flexible – LA version is ideal for amplifying any target up to 30 kb from DNA extracted from human, animal and plant samples

Data Sheet:  KlenTaq, KlenTaq LA